Anti-Neurofilamentmedium polypeptide (NF-M) Antilichaam

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70 kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)

Our Anti-Neurofilament medium polypeptide (NF-M) chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament medium polypeptide (NF-M), and is igy preparation. It is validated for use in IF, ICC, IHC, WB.

Our Anti-Neurofilament medium polypeptide (NF-M) chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament medium polypeptide (NF-M), and is IgY preparation. It is validated for use in IF, ICC, IHC, WB.

NF-M; NFM; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm

P07197

Bovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, and Rat

This antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.

Neurofilament medium polypeptide (NF-M)

Unconjugated

IF, ICC, IHC, WB

IgY preparation

Lyophilized IgY preparation, with sodium azide.

~145 kDa for rodent and ~160 kDa for human and cow

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

1.4305555555555556

1:2000-1:5000

Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.

1.4305555555555556

Chicken

Lyophilized

IgY

Human