Anti-NLRC4 (Ser-533), Fosfospecifiek antilichaam

The nucleotide-binding oligomerization domain (NOD) -like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCδ following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCδ phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.

Our NLRC4 (Ser-533) rat monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NLRC4 (Ser-533) and is antigen affinity purified. It is great for use in ELISA, WB.

Our NLRC4 (Ser-533) rat rat monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NLRC4 (Ser-533) and is antigen affinity purified. It is great for use in WB.

CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4

NLRC4

Q3UP24

Ser-533

Human, Mouse, and Rat

Clone M549 was generated from a phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.

NLRC4 (Ser-533)

M549

Unconjugated

ELISA, WB

Immunology

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

110

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment.

Western blots performed on each lot.

Rat

Liquid

N.T.

Mouse

Quality Control: WB

AB_3068479