Anti-NOX2/gp91phox/CYBB Antilichaam Picoband® 31.0647 Vervoeging

NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling) .

Cytochrome b-245 heavy chain;1.-.-.-; CGD91-phox; Cytochrome b (558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome; CYBB; NOX2

CYBB

1536

P04839

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human NOX2, identical to the related rat and mouse sequences.

Detected in neutrophils (at protein level) . .

Flow Cytometry

Adaptive Immunity, Cancer, Cancer Metabolism, Immunology, Metabolism, T Cells

Immunogen affinity purified.

Liquid

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome b (558) -positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet. 115: 418-427, 2004. 2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the beta-subunit of cytochrome b (558) leading to X-linked chronic granulomatous disease. Blood 77: 2482-2487, 1991. 3. Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease) .

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

65336 MW

No cross reactivity with other proteins.

6

Cytochrome b-245 heavy chain

Cell membrane; Multi-pass membrane protein.

Contains 1 FAD-binding FR-type domain.

Cytochrome b-245 heavy chain

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.