Anti-NSUN3 Antibody Picoband® 31.0550 Vervoeging

NSUN3 (NOL1/NOP2/Sun domain family, member 3), also known as MST077, is a 340 amino acid protein that belongs to the methyltransferase superfamily and is thought to function as an S-adenosyl-L-methionine-dependent methyltransferase. The gene encoding NSUN3 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

NSUN3

63899

Q9H649

Human, Monkey

No cross-reactivity with other proteins.

E.coli-derived human NSUN3 recombinant protein (Position: K12-E310) .

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Flow Cytometry

Cell Biology

Immunogen affinity purified.

Liquid

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

1. Haag, S., Sloan, K. E., Ranjan, N., Warda, A. S., Kretschmer, J., Blessing, C., Hubner, B., Seikowski, J., Dennerlein, S., Rehling, P., Rodnina, M., Hobartner, C., Bohnsack, M. T. NSUN3 and ABH1 modify the wobble position of mt-tRNA (Met) to expand codon recognition in mitochondrial translation. EMBO J. 35: 2104-2119, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/25/2017. 3. Nakano, S., Suzuki, T., Kawarada, L., Iwata, H., Asano, K., Suzuki, T. NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA (Met) . Nature Chem. Biol. 12: 546-551, 2016.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

24145 MW

6

Kelch repeat and BTB domain containing 2

Cul3-RING ubiquitin ligase complex.

Transmembrane protein 240

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.