Anti-Occludin/OCLN Antibody Picoband® fluoro647 Vervoeging

Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Occludin; OCLN

OCLN

100506658

Q16625

Human, Mouse, Rat

No cross-reactivity with other proteins

E.coli-derived human Occludin/OCLN recombinant protein (Position: S358-Q520) . Human Occludin/OCLN shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat Occludin/OCLN, respectively.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Ando-Akatsuka, Y., Saitou, M., Hirase, T., Kishi, M., Sakakibara, A., Itoh, M., Yonemura, S., Furuse, M., Tsukita, S. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133: 43-47, 1996. 2. Argaw, A. T., Gurfein, B. T., Zhang, Y., Zameer, A., John, G. R. VEGF-mediated disruption of endothelial CLN-5 promotes blood-brain barrier breakdown. Proc. Nat. Acad. Sci. 106: 1977-1982, 2009. 3. Barrios-Rodiles, M., Brown, K. R., Ozdamar, B., Bose, R., Liu, Z., Donovan, R. S., Shinjo, F., Liu, Y., Dembowy, J., Taylor, I. W., Luga, V., Przulj, N., Robinson, M., Suzuki, H., Hayashizaki, Y., Jurisica, I., Wrana, J. L. High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307: 1621-1625, 2005.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

59 kDa

6

Occludin

Occludin

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.