Anti-Omalizumab Neutraliserende Antibody ELISA kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Omalizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human IgE and then pipetted into the wells. Anti-Omalizumab Neutralizing Antibody in the sample competitively binds to Omalizumab with the biotin-labeled Human IgE. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Omalizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Omalizumab

IGE25, olizumab, pSVIE26, rhuMab-E25, CAS: 242138-07-4

Colorimetric

Quantitative

Plasma, Serum

156.25 - 10,000 ng/mL

80-120%

82.6 ng/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.