Anti-OS9 Antibody Picoband® fluoro488 Vervoeging

Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Erf1; Protein Cl1; TB3-1; ETF1; ERF1; RF1; SUP45L1

OS9

10956

Q13438

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human OS9 recombinant protein (Position: Q56-E635) .

Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Flow Cytometry

Adapters, Cell Type Marker, Intracellular Signaling, Neuron Marker, Neuroscience, Neurotransmission, Synapse Marker

Immunogen affinity purified.

Liquid

S the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.

1. Baek, J. H., Mahon, P. C., Oh, J., Kelly, B., Krishnamachary, B., Pearson, M., Chan, D. A., Giaccia, A. J., Semenza, G. L. OS-9 interacts with hypoxia-inducible factor 1-alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1-alpha. Molec. Cell 17: 503-512, 2005. 2. Bhamidipati, A., Denic, V., Quan, E. M., Weissman, J. S. Exploration of the topological requirements of ERAD identifies Yos9p as a lectin sensor of misfolded glycoproteins in the ER lumen. Molec. Cell 19: 741-751, 2005. 3. Friedmann, E., Salzberg, Y., Weinberger, A., Shaltiel, S., Gerst, J. E. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER) -Golgi transport of GPI-anchored proteins. J. Biol. Chem. 277: 35274-35281, 2002.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

90314 MW

6

Eukaryotic translation termination factor 1

Cytoplasm.

Chromodomain-helicase-DNA-binding protein 2

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.