Anti-Osteopontin/SPP1 Antibody Picoband® 31.0550 Vervoeging

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1) . Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP, OPN; PSEC0156

SPP1

6696

P10451

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin, different from the related mouse sequence by six amino acids.

Bone. Found in plasma.

Flow Cytometry

Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures, Tumor Biomarkers

Immunogen affinity purified.

Liquid

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

1. Beck, G. R., Jr.; Zerler, B.; Moran, E. Phosphate is a specific signal for induction of osteopontin gene expression. Proc. Nat. Acad. Sci. 97: 8352-8357, 2000. 2. Kohri, K.; Suzuki, Y.; Yoshida, K.; Yamamoto, K.; Amasaki, N.; Yamate, T.; Umekawa, T.; Iguchi, M.; Sinohara, H.; Kurita, T. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. Biochem. Biophys. Res. Commun. 184: 859-864, 1992. 3. Fisher, L. W.; McBride, O. W.; Termine, J. D.; Young, M. F. Human bone sialoprotein: deduced protein sequence and chromosomal localization. J. Biol. Chem. 265: 2347-2351, 1990.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

35423 MW

No cross reactivity with other proteins.

6

Osteopontin

Secreted.

Belongs to the osteopontin family.

Osteopontin

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.