Anti-p27 KIP 1/CDKN1B Antibody Picoband® 0,00647 Vervoeging

Cyclin-dependent kinase inhibitor 1B (p27KIP1), also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kipfamily of cyclin dependent kinase (Cdk) inhibitor proteins. It is mapped to 12p13.1. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of p27KIP1 is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B; KIP1

CDKN1B

1027

P46527

Human

No cross-reactivity with other proteins

E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198) . Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Flow Cytometry

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. .

1. Polyak, K., Lee, M.-H., Erdjument-Bromage, H., Koff, A., Roberts, J. M., Tempst, P., Massague, J. Cloning of p27 (Kip1), a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78: 59-66, 1994. 2. Hauck, L., Harms, C., An, J., Rohne, J., Gertz, K., Dietz, R., Endres, M., von Harsdorf, R.Protein kinase CK2 links extracellular growth factor signaling with the control of p27 (Kip1) stability in the heart. Nature Med. 14: 315-324, 2008. Note: Erratum: Nature Med. 14: 585 only, 2008.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

22073 MW

No cross reactivity with other proteins.

6

Cyclin-dependent kinase inhibitor 1B

Nucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) . .

Belongs to the CDI family.

Cyclin-dependent kinase inhibitor 1B

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.