Anti-p27Kip1 CDKN1B Antilichaam (Monoclonal, DCS-72)

P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.

Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Cyclin dependent kinase inhibitor ; Cyclin kinase inhibitor ; Cyclin-dependent kinase inhibitor 1B ; P27 kip1 ; P27kip1 ; Protein Cdkn1b ; Cdkn1b ; p27Kip1 ; rCG_29991

CDKN1B

Human (1027), Mouse (12576), Rat (83571)

O08769

Human, Mouse, Rat

No cross-reactivity with other proteins

Recombinant rodent p27Kip1 protein.

Clone: DCS-72

CDKN1B: Expressed in all tissues tested. Highestlevels in skeletal muscle, lowest in liver and kidney.

ICC, WB

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling

Ascites

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Lyophilized

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

CDKN1B: Important regulator of cell cycle progression. Involvedin G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2complexes. Forms a complex with cyclin type D-CDK4 complexes andis involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or anactivator of cyclin type D-CDK4 complexes depending on itsphosphorylation state and/or stoichometry.

1. Chang, B.; Zheng, S. L.; Isaacs, S. D.; Wiley, K. E.; Turner, A.; Li, G.; Walsh, P. C.; Meyers, D. A.; Isaacs, W. B.; Xu, J. : A polymorphism in the CDKN1B gene is associated with increased risk of hereditary prostate cancer. Cancer Res. 64: 1997-1999, 2004. 2. Malek, N. P.; Sundberg, H.; McGrew, S.; Nakayama, K.; Kyriakidis, T. R.; Roberts, J. M. : A mouse knock-in model exposes sequential proteolytic pathways that regulate p27 (Kip1) in G1 and S phase. Nature 413: 323-327, 2001. 3. Mitsuhashi, T.; Aoki, Y.; Eksioglu, Y. Z.; Takahashi, T.; Bhide, P. G.; Reeves, S. A.; Caviness, V. S., Jr. : Overexpression of p27 (Kip1) lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc. Nat. Acad. Sci. 98: 6435-6440, 2001.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=MA1076

22139 MW

18 kDa

No cross reactivity with other proteins.

Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CDKN1B: Nucleus. Cytoplasm. Endosome Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates tothe cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results intranslocation to the cytoplasm and cell cycle progression.Phosphorylation on Ser-10 facilitates nuclear export. Translocatesto the nucleus on phosphorylation of Tyr-88 and Tyr-89.Colocalizes at the endosome with SNX6; this leads to lysosomaldegradation (By similarity) . |Cdkn1b: Nucleus. Cytoplasm. Endosome. Note: Nuclearand cytoplasmic in quiescent cells. AKT- or RSK-mediatedphosphorylation on Thr-197, binds 14-3-3, translocates to thecytoplasm and promotes cell cycle progression. Mitogen-activatedUHMK1 phosphorylation on Ser-10 also results in translocation tothe cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus onphosphorylation of Tyr-88 and Tyr-89 (By similarity) . Colocalizesat the endosome with SNX6; this leads to lysosomal degradation.

Belongs to the CDI family.

Cyclin-dependent kinase inhibitor 1B

Mouse IgG1

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.