Anti-P2X4/P2RX4 Antibody Picoband® fluoro550 Vervoeging

Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. P2X4 receptor in hyperactive microglia is necessary for tactile allodynia after nerve injury and is sufficient to produce tactile allodynia in normal animals. P2X4 recetors have beeb implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic strengthening, and activating of the inflammasome in response to injury.

Heat shock factor protein 2; HSF 2; Heat shock transcription factor 2; HSTF 2; HSF2; HSTF2

P2RX4

5025

Q99571

Human

No cross-reactivity with other proteins.

E.coli-derived human P2X4/P2RX4 recombinant protein (Position: Q111-E249) .

Flow Cytometry

Epigenetics and Nuclear Signaling, Transcription

Immunogen affinity purified.

Liquid

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

1. Adrian, K., Bernhard, M. K., Breitinger, H.-G., Ogilvie, A. Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells. Biochim. Biophys. Acta 1492: 127-138, 2000. 2. Kawate, T., Michel, J. C., Birdsong, W. T., Gouaux, E. Crystal structure of the ATP-gated P2X4 ion channel in the closed state. Nature 460: 592-598, 2009. 3. Wilson, S. G., Adam, G., Langdown, M., Reneland, R., Braun, A., Andrew, T., Surdulescu, G. L., Norberg, M., Dudbridge, F., Reed, P. W., Sambrook, P. N., Kleyn, P. W., Spector, T. D. Linkage and potential association of obesity-related phenotypes with two genes on chromosome 12q24 in a female dizygous twin cohort. Europ. J. Hum. Genet. 14: 340-348, 2006. 4. Yamamoto, K., Sokabe, T., Matsumoto, T., Yoshimura, K., Shibata, M., Ohura, N., Fukuda, T., Sato, T., Sekine, K., Kato, S., Isshiki, M., Fujita, T., Kobayashi, M., Kawamura, K., Masuda, H., Kamiya, A., Ando, J. Impaired flow-dependent control of vascular tone and remodeling in P2X4-deficient mice. Nature Med. 12: 133-137, 2006.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Heat shock transcription factor 2

Cytoplasm.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.