Anti-p53R2/RRM2B Antilichaam Picoband® fluoro488 Vervoeging

RRM2B (Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al. (2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al. (2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.

Ribonucleoside-diphosphate reductase subunit M2 B;1.17.4.1; TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; RRM2B; P53R2

RRM2B

50484

Q7LG56

Rat, Mouse, Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2, identical to the related rat and mouse sequences.

Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. .

Flow Cytometry

DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Oncoproteins/Suppressors, p53 Pathway, Pathways and Processes, Tumor Suppressors

Immunogen affinity purified.

Liquid

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. .

1. Kimura, T., Takeda, S., Sagiya, Y., Gotoh, M., Nakamura, Y., Arakawa, H. Impaired function of p53R2 in Rrm2b-null mice causes severe renal failure through attenuation of dNTP pools. Nature Genet. 34: 440-445, 2003. 2. Kollberg, G., Darin, N., Benan, K., Moslemi, A.-R., Lindal, S., Tulinius, M., Oldfors, A., Holme, E. A novel homozygous RRM2B missense mutation in association with severe mtDNA depletion. Neuromusc. Disord. 19: 147-150, 2009. 3. Takata, A., Kato, M., Nakamura, M., Yoshikawa, T., Kanba, S., Sano, A., Kato, T. Exome sequencing identifies a novel missense variant in RRM2B associated with autosomal recessive progressive external ophthalmoplegia. Genome Biol. 12: R92, 2011.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

40737 MW

No cross reactivity with other proteins.

6

Ribonucleoside-diphosphate reductase subunit M2 B

Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.

Belongs to the ribonucleoside diphosphate reductase small chain family.

Ribonucleoside-diphosphate reductase subunit M2 B

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.