Anti-PAK3 Antibody Picoband® 31.0550 Vervoeging

Serine/threonine-protein kinase PAK 3is anenzymethat in humans is encoded by thePAK3gene. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47) . Alternatively spliced transcript variants encoding different isoforms have been identified.

Eosinophil cationic protein; ECP; Ribonuclease 3; RNase 3; RNASE3; ECP; RNS3

PAK3

5063

O75914

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human PAK3 recombinant protein (Position: M1-D263) .

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Flow Cytometry

2339, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

Immunogen affinity purified.

Liquid

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

1. Entrez Gene: PAK3 p21 (CDKN1A) -activated kinase 3. 2. Allen KM, Gleeson JG, Bagrodia S, Partington MW, MacMillan JC, Cerione RA, Mulley JC, Walsh CA (Oct 1998) . PAK3 mutation in nonsyndromic X-linked mental retardation. Nat Genet 20 (1) : 25–30. 3. Donnelly AJ, Partington MW, Ryan AK, Mulley JC (Nov 1996) . Regional localisation of two non-specific X-linked mental retardation genes (MRX30 and MRX31) . Am J Med Genet 64 (1) : 113–20.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

64406 MW

6

Ribonuclease A family member 3

Secreted. Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

Transcription factor E2F3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.