Anti-Paxilline/PXN Antibody Picoband® fluoro488 Vervoeging

PXN (Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al. (1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein– protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.

Paxillin; PXN

PXN

5829

P49023

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin, identical to the related mouse and rat sequences.

Flow Cytometry

Adapters, Cancer, Cytoplasmic, Cytoskeleton/ECM, Extracellular Matrix, Signal Transduction, Structures

Immunogen affinity purified.

Liquid

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) .

1. Fernandez-Valle, C., Tang, Y., Ricard, J., Rodenas-Ruano, A., Taylor, A., Hackler, E., Biggerstaff, J., Iacovelli, J.Paxillin binds schwannomin and regulates its absorbance-dependent localization and effect on cell morphology.Nature Genet. 31: 354-362, 2002. 2. Salgia, R., Li, J.-L., Lo, S. H., Brunkhorst, B., Kansas, G. S., Sobhany, E. S., Sun, Y., Pisick, E., Hallek, M., Ernst, T., Tantravahi, R., Chen, L. B., Griffin, J. D.Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210 (BCR/ABL) .J. Biol. Chem. 270: 5039-5047, 1995. 3. Turner, C. E., Glenney, J. R., Jr., Burridge, K.Paxillin: a new vinculin-binding protein present in focal adhesions.J. Cell Biol. 111: 1059-1068, 1990.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

64505 MW

No cross reactivity with other proteins.

6

Paxillin

Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. .

Belongs to the paxillin family.

Paxillin

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.