Anti-PC1/3/PCSK1 Antibody Picoband® fluoro647 Vervoeging

PCSK1 (Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al. (1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al. (1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al. (2003) concluded that human intestinal absorptive function is dependent on PC1 activity.

Neuroendocrine convertase 1; NEC 1;3.4.21.93; Prohormone convertase 1; Proprotein convertase 1; PC1; PCSK1; NEC1

PCSK1

5122

P29120

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3, identical to the related mouse and rat sequences.

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. .

Flow Cytometry

Cancer, Cancer Metabolism, Cell Biology, Endocrine Metabolism, Growth Factors/Hormones, Hormone Biosynthesis, Metabolic Signaling Pathway, Metabolism, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Immunogen affinity purified.

Liquid

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

1. Jackson, R. S., Creemers, J. W. M., Farooqi, I. S., Raffin-Sanson, M.-L., Varro, A., Dockray, G. J., Holst, J. J., Brubaker, P. L., Corvol, P., Polonsky, K. S., Ostrega, D., Becker, K. L., and 10 others. Small-intestinal dysfunction accompanies the complex endocrinopathy of human proprotein convertase 1 deficiency. J. Clin. Invest. 112: 1550-1560, 2003. 2. Ohagi, S., Sakaguchi, H., Sanke, T., Tatsuta, H., Hanabusa, T., Nanjo, K. Human prohormone convertase 3 gene: exon-intron organization and molecular scanning for mutations in Japanese subjects with NIDDM. Diabetes 45: 897-901, 1996. 3. Seidah, N. G., Mattei, M. G., Gaspar, L., Benjannet, S., Mbikay, M., Chretien, M. Chromosomal assignments of the genes for neuroendocrine convertase PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2) to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region) . Genomics 11: 103-107, 1991.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

84152 MW

No cross reactivity with other proteins.

6

Neuroendocrine convertase 1

Cytoplasmic vesicle, secretory vesicle. Localized in the secretion granules.

Belongs to the peptidase S8 family. Furin subfamily.

Neuroendocrine convertase 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.