Anti-PCNA Konijn Monoklonale Antilichaam

Boster Bio Anti-PCNA Rabbit Monoclonal Antibody catalog # M00125-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA

PCNA

5111

P12004

Human, Mouse, Rat

A synthesized peptide derived from human PCNA

Clone: DO-16

Ubiquitously expressed. .

WB, IHC, ICC, IF, IP, Flow Cytometry

Cell Biology, Cell Cycle, Cell Division, Cell Type Markers, Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, DNA Synthesis, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Tumor Antigens, Tumor Biomarkers

Affinity-chromatography

0.5mg/ml

Liquid

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902) . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M00125-1

28769 MW

36 kDa

Proliferating cell nuclear antigen

Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902) . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .

P2X purinoceptor 1

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.