Anti-PDF Antibody Picoband®

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.

Boster Bio Anti-PDF Antibody Picoband® catalog # A00304-1. Tested in ELISA, IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

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PDF

64146

Q9HBH1

Human, Mouse, Rat

No cross reactivity with other proteins.

E.coli-derived human PDF recombinant protein (Position: H51-Q199) . Human PDF shares 82.4% amino acid (aa) sequence identity with mouse PDF.

Expressed in all tissues.

WB, IHC, IF, Flow Cytometry, ELISA

Signal Transduction, Signaling Pathway, Small G Proteins

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

1. Escobar-Alvarez, S., Goldgur, Y., Yang, G., Ouerfelli, O., Li, Y., Scheinberg, D. A. Structure and activity of human mitochondrial peptide deformylase, a novel cancer target. J. Molec. Biol. 387: 1211-1228, 2009. 2. Giglione, C., Serero, A., Pierre, M., Boisson, B., Meinnel, T. Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms. EMBO J. 19: 5916-5929, 2000. 3. Lee, M. D., Antczak, C., Li, Y., Sirotnak, F. M., Bornmann, W. G., Scheinberg, D. A. A new human peptide deformylase inhibitable by actinonin. Biochem. Biophys. Res. Commun. 312: 309-315, 2003.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A00304-1

52588 MW

25 kDa

6

Serine/threonine-protein kinase AtPK1/AtPK6

Cytoplasm. Nucleus.

Serine/threonine-protein kinase AtPK1/AtPK6

IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.