Anti-PDP1/PDP Antibody Picoband® fluoro550 Vervoeging

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB

PDP1

54704

Q9P0J1

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human PDP1/PDP recombinant protein (Position: S37-E537) .

Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level) .

Flow Cytometry

Amino Acid Metabolism, Amino Acids, Cofactors, Vitamins/Minerals, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Immunogen affinity purified.

Liquid

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.

1. Bedoyan, J. K., Hecht, L., Zhang, S., Tarrant, S., Bergin, A., Demirbas, D., Yang, E., Shin, H. K., Grahame, G. J., DeBrosse, S. D., Hoppel, C. L., Kerr, D. S., Berry, G. T. A novel null mutation in the pyruvate dehydrogenase phosphatase catalytic subunit gene (PDP1) causing pyruvate dehydrogenase complex deficiency. JIMD Rep. 48: 26-35, 2019. 2. Cameron, J. M., Maj, M., Levandovskiy, V., Barnett, C. P., Blaser, S., MacKay, N., Raiman, J., Feigenbaum, A., Schulze, A., Robinson, B. H. Pyruvate dehydrogenase phosphatase 1 (PDP1) null mutation produces a lethal infantile phenotype. Hum. Genet. 125: 319-326, 2009. 3. Huang, B., Gudi, R., Wu, P., Harris, R. A., Hamilton, J., Popov, K. M. Isoenzymes of pyruvate dehydrogenase phosphatase: DNA-derived amino acid sequences, expression, and regulation. J. Biol. Chem. 273: 17680-17688, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

48608 MW

6

Phosphopantothenoylcysteine synthetase

Cytosol.

Serine protease HTRA3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.