Anti-Pembrolizumab Neutraliseren Antibody ELISA kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD279 antigen and then pipetted into the wells. Anti-Pembrolizumab Neutralizing Antibody in the sample competitively binds to Pembrolizumab with the biotin-labeled Human CD279 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pembrolizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

MK-3475, CAS: 1374853-91-4

Used for the quantitative determination of Anti-Pembrolizumab Neutralizing Antibody concentration in serum and plasma.

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

2-8 °C

For Research Use Only.