Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband®

PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23.  The PRDX3 gene is mapped on 10q26.11. Expression of  PRDX3  is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire  PRDX3  genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that  PRDX3  is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that  PRDX3  is a MYC target gene that is required to maintain normal mitochondrial function.

Boster Bio Anti-Peroxiredoxin 3/PRDX3 Antibody catalog # PA1835. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Thioredoxin-dependent peroxide reductase, mitochondrial;1.11.1.15; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; PRDX3; AOP1

PRDX3

10935

P30048

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 3, different from the related mouse and rat sequences by three amino acids.

IF, IHC, ICC, WB, Flow Cytometry

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cell Biology, Cell Death, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. .

1. Chiribau, C. B., Cheng, L., Cucoranu, I. C., Yu, Y.-S., Clempus, R. E., Sorescu, D. FOXO3A regulates peroxiredoxin III expression in human cardiac fibroblasts. J. Biol. Chem. 283: 8211-8217, 2008. 2. Nemoto, Y., Yamamoto, T., Takada, S., Matsui, Y., Obinata, M. Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 91: 261-265, 1990. 3. Wonsey, D. R., Zeller, K. I., Dang, C. V. The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proc. Nat. Acad. Sci. 99: 6649-6654, 2002.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1835

27693 MW

26 kDa

No cross reactivity with other proteins.

6

Thioredoxin-dependent peroxide reductase, mitochondrial

Mitochondrion.

Belongs to the AhpC/TSA family.

Thioredoxin-dependent peroxide reductase, mitochondrial

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.