Anti-Phospholipase A2/PLA2G4A Antilichaam Picoband® 31.0647 Vervoeging

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-ed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue (s) . PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995) .

Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2;3.1.1.4; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase;3.1.1.5; PLA2G4A; CPLA2, PLA2G4

PLA2G4A

5321

P47712

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related rat and mouse sequences by one amino acid.

Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.

Flow Cytometry

Signaling Pathway, Vascular Inflammation

Immunogen affinity purified.

Liquid

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

1. Sharp, J. D., Pickard, R. T., Chiou, X. G., Manetta, J. V., Kovacevic, S., Miller, J. R., Varshavsky, A. D., Roberts, E. F., Strifler, B. A., Brems, D. N., Kramer, R. M. Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 269: 23250-23254, 1994. 2. Skorecki, K. L. Personal Communication. Toronto, Canada 4/19/1995. 3. Tay, A., Simon, J. S., Squire, J., Hamel, K., Jacob, H. J., Skorecki, K. Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 26: 138-141, 1995.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

85239 MW

No cross reactivity with other proteins.

6

Cytosolic phospholipase A2

Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.

Contains 1 C2 domain.

Cytosolic phospholipase A2

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.