Anti-Phosphotyrosine Antilichaam

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.

Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is protein a purified. It is great for use in ELISA, WB, ICC, IP.

Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is Protein A purified. It is great for use in WB, ICC, IP.

Phosphotyrosine mAb

P-Tyr

Chicken, Human, Mouse, and Rat

Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.

Anti-Phosphotyrosine

M375

Unconjugated

ELISA, WB, ICC, IP

Cell Signaling, Kinase Signaling

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

1:100

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG2b

Human

Quality Control: WB

AB_1944435