Anti-Pirh2/RCHY1 Antibody Picoband® fluoro488 Vervoeging

RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3

RCHY1

25898

Q96PM5

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human Pirh2/RCHY1 recombinant protein (Position: Q17-Q250) .

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Flow Cytometry

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Immunogen affinity purified.

Liquid

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.

1. Fedorova, O. , Gudovich, A. , Daks, A. , Baidyuk, E. , & Barlev, N. A. . (2021) . Regulation of autophagy flux by e3 ubiquitin ligase pirh2 in lung cancer. Biochemical and Biophysical Research Communications, 563, 119-125. 2. Yifeng, Bao, Xue, Wu, Debin, & Yuan, et al. (2017) . High expression of pirh2 is associated with poor prognosis in glioma. Cellular and molecular neurobiology. 3. Yang, ShuyunChen, YingyingSun, FulanNi, QichaoWang, HuaHuang, YeqingZhang, ChunhuiLiu, KunWang, ShuoQiu, JianXu, ZhenHua, LuHe, Zhixian. (2016) . Downregulated pirh2 can decrease the proliferation of breast cancer cells. Archives of medical research, 47 (3) .

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

RAB11B, member RAS oncogene family

Recycling endosome membrane, Lipid-anchor, Synaptic vesicle membrane, Phagosome membrane.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.