Anti-PIWIL4/PIWI Antibody Picoband® fluoro594 Vervoeging

PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838) . There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77% aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.

PR domain zinc finger protein 5; PR domain-containing protein 5; PRDM5; PFM2

PIWIL4

143689

Q7Z3Z4

Human

No cross-reactivity with other proteins.

E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749) .

Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Flow Cytometry

Cell Adhesion Proteins, ECM Proteins, Neuroscience

Immunogen affinity purified.

Liquid

Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May cause G2/M arrest and apoptosis in cancer cells.

1. Carmell, M. A., Girard, A., van de Kant, H. J. G., Bourc'his, D., Bestor, T. H., de Rooij, D. G., Hannon, G. J. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev. Cell 12: 503-514, 2007. 2. De Fazio, S., Bartonicek, N., Di Giacomo, M., Abreu-Goodger, C., Sankar, A., Funaya, C., Antony, C., Moreira, P. N., Enright, A. J., O'Carroll, D. The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature 480: 259-263, 2011. 3. Sasaki, T., Shiohama, A., Minoshima, S., Shimizu, N. Identification of eight members of the Argonaute family in the human genome. Genomics 82: 323-330, 2003.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

104553 MW

6

PR/SET domain 5

Nucleus.

AP-2 complex subunit beta

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.