Anti-PKC (α, β, γ) Antilichaam

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V) . The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

Our PKC (α, β, γ) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat PKC (α, β, γ) and is protein g purified. It is great for use in ELISA, WB, ICC.

Our PKC (α, β, γ) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat PKC (α, β, γ) and is Protein G purified. It is great for use in WB, ICC.

PKCalpha, PKCbeta, PKCgamma

PRKCA

P05129

Chicken, Human, Mouse, and Rat

Clone (M499) was generated from a recombinant human PKCγ that included amino acids residues 499-697.

PKC (α, β, γ)

M499

Unconjugated

ELISA, WB, ICC

Cell Signaling, Kinase Signaling, Oncoproteins

Protein G Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

80-82

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein G purified culture supernatant.

1:1000

This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKCα, PKCβ, and PKCγ on SDS-PAGE immunoblots of mouse brain lysates. Similar results were observed in human and mouse lysates. This antibody detects recombinant PKCα, PKCβ1, and PKCγ, and possibly detects other PKC isoforms as well.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG2a

Human

Quality Control: WB

AB_3068486