Anti-Plexine A1 (Sema Domain) Antilichaam

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.<

Our Plexin A1 (Sema Domain) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin A1 (Sema Domain) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IHC, IP.

Our Plexin A1 (Sema Domain) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin A1 (Sema Domain) and is antigen affinity purified. It is great for use in WB, ICC, IHC, IP.

PLXN1, NOV, Sema3A

PLXNA1

Q9UIW2

Human, Mouse, and Rat

Clone M535 was generated from a Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

Plexin A1 (Sema Domain)

M535

Unconjugated

ELISA, WB, ICC, IHC, IP

Cell Signaling, Cytoskeletal Regulation, Neurogenesis and Development

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

GOmAb

220

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:100

1:500

1:50

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_3068487