Anti-PMS2 Konijn Monoklonale Antilichaam

Boster Bio Anti-PMS2 Rabbit Monoclonal Antibody catalog # M01028-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Mismatch repair endonuclease PMS2 ;3.1.-.-; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; PMS2 ; PMSL2

PMS2

5395

P54278

Human

A synthesized peptide derived from human PMS2

Clone: AEH-16

Ubiquitous. .

WB, IHC, ICC, IF, IP, Flow Cytometry

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Affinity-chromatography

0.5mg/ml

Liquid

Component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M01028-1

95797 MW

120 kDa

Mismatch repair endonuclease PMS2

Nucleus .

Glycogen synthase kinase-3 beta (gsk3b)

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.