Anti-POLR1E Antibody Picoband®

Predicted to enable DNA binding activity and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription initiation at RNA polymerase I promoter. Located in fibrillar center and nucleoplasm. Part of RNA polymerase I complex.

Boster Bio Anti-POLR1E Antibody Picoband® catalog # A11077-2. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

POLR1E; PAF53; PRAF1; DNA-directed RNA polymerase I subunit RPA49; RNA polymerase I subunit A49; DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53

POLR1E

64425

Q9GZS1

Human

No cross-reactivity with other proteins

E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) . Human POLR1E shares 78.9% amino acid (aa) sequence identity with mouse POLR1E.

WB, ICC/IF, IP, ELISA

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

1. Hanada, K., Song, C. Z., Yamamoto, K., Yano, K., Maeda, Y., Yamaguchi, K., Muramatsu, M. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription. EMBO J. 15: 2217-2226, 1996. 2. Kong, R., Zhang, L., Hu, L., Peng, Q., Han, W., Du, X., Ke, Y. hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF) . J. Biol. Chem. 286: 7139-7148, 2011. 3. McNamar, R., Abu-Adas, Z., Rothblum, K., Knutson, B. A., Rothblum, L. I. Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains. J. Biol. Chem. 294: 19907-19922, 2019.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A11077-2

47 kDa

47 kDa

RNA polymerase I subunit E

DNA-directed RNA polymerase I subunit RPA49

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.