Anti-POLR1E Antibody Picoband® APC-vervoeging

Predicted to enable DNA binding activity and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription initiation at RNA polymerase I promoter. Located in fibrillar center and nucleoplasm. Part of RNA polymerase I complex.

POLR1E; PAF53; PRAF1; DNA-directed RNA polymerase I subunit RPA49; RNA polymerase I subunit A49; DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53

POLR1E

64425

Q9GZS1

Human

No cross-reactivity with other proteins

E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) . Human POLR1E shares 78.9% amino acid (aa) sequence identity with mouse POLR1E.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Hanada, K., Song, C. Z., Yamamoto, K., Yano, K., Maeda, Y., Yamaguchi, K., Muramatsu, M. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription. EMBO J. 15: 2217-2226, 1996. 2. Kong, R., Zhang, L., Hu, L., Peng, Q., Han, W., Du, X., Ke, Y. hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF) . J. Biol. Chem. 286: 7139-7148, 2011. 3. McNamar, R., Abu-Adas, Z., Rothblum, K., Knutson, B. A., Rothblum, L. I. Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains. J. Biol. Chem. 294: 19907-19922, 2019.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

47 kDa

6

RNA polymerase I subunit E

DNA-directed RNA polymerase I subunit RPA49

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.