Anti-Polycomb complex eiwit BMI-1 Bmi1 Antibody Picoband® fluoro550 Vervoeging

BMI1 (BMI1 polycomb ring finger oncogene), also known as RNF51,   is a  protein  which in humans is encoded by the  BMI1  gene. The  Bmi1  gene is highly conserved in evolution as indicated by zoo blot hybridization with  Bmi1  probes corresponding to the protein-encoding domain. By fluorescence in situ hybridization, the human  BMI1  gene is assigned to chromosome 10p13. BMI1  has a key role in regulating the proliferative activity of normal stem and progenitor cells. Most importantly, they provided evidence that the proliferative potential of leukemic stem and progenitor cells lacking  BMI1  is compromised because they eventually undergo proliferation arrest and show signs of differentiation and apoptosis, leading to transplant failure of the leukemia. Complementation studies showed that  BMI1  completely rescues these proliferative defects. Deletion analysis showed that the RING finger and helix-turn-helix domains of  BMI1  were required for life span extension and repression of the tumor suppressor p16 (INK4) . BMI1  selectively extended the life span of these cultures. Confocal microscopy showed that  BMI1  transiently colocalized with centromeres during interphase in HeLa cells.

Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; BMI1; PCGF4, RNF51

BMI1

648

P35226

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human Bmi1, different from the related mouse sequence by three amino acids.

Flow Cytometry

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation,2339, Chromatin Remodeling, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Stem Cells, Transcription

Immunogen affinity purified.

Liquid

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. .

1. Alkema, M. J., van der Lugt, N. M. T., Bobeldijk, R. C., Berns, A., van Lohuizen, M. Transformation of axial skeleton due to overexpression of bmi-1 in transgenic mice. Nature 374: 724-727, 1995. 2. Chagraoui, J., Niessen, S. L., Lessard, J., Girard, S., Coulombe, P., Sauvageau, M., Meloche, S., Sauvageau, G. E4F1: a novel candidate factor for mediating BMI1 function in primitive hematopoietic cells. Genes Dev. 20: 2110-2120, 2006. 3. Leung, C., Lingbeek, M., Shakhova, O., Liu, J., Tanger, E., Saremaslani, P., van Lohuizen, M., Marino, S. Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas. Nature 428: 337-341, 2004.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

36949 MW

No cross reactivity with other proteins.

6

Polycomb complex protein BMI-1

Nucleus. Cytoplasm.

Contains 1 RING-type zinc finger.

Polycomb complex protein BMI-1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.