Anti-Polyubiqutin-B Antilichaam

Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a) .

Our Anti-Polyubiqutin-B mouse monoclonal primary antibody detects bovine, c. elegans, chicken, drosophila, and human Polyubiqutin-B , and is igg. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

Our Anti-Polyubiqutin-B mouse monoclonal primary antibody detects bovine, C. elegans, chicken, drosophila, and human Polyubiqutin-B, and is IgG. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

RPS27A; UBA52; UBB; UBC; Polyubiquitin-B; Polyubiquitin-C

P0CG47

Bovine, C. elegans, Chicken, Drosophila, and Human

Raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.

Polyubiqutin-B

Ubi-1

Unconjugated

IHC-Frozen, IHC-Paraffin-embedded, WB

IgG

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

8.5 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Protein G purified

0.3888888888888889

1:500-1:1000

The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin. Hu, Bov, Chk, Drosophila, and C. elegans.

Mouse

Lyophilized

IgG1

Human