Anti-PRAS40 AKT1S1 Monoklonale Antilichaam

Boster Bio Anti-PRAS40 AKT1S1 Monoclonal Antibody catalog # M03629-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Proline-rich AKT1 substrate 1;40 kDa proline-rich AKT substrate; AKT1S1 ; PRAS40

AKT1S1

84335

Q96B36

Human, Mouse, Rat

A synthesized peptide derived from human PRAS40 Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.

Clone: ACHD-1

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293) . .

WB, IP

Cell Biology, Cell Cycle, Intracellular Signaling, Kinases, Kinases/Phosphatases, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Affinity-chromatography

0.5mg/ml

Liquid

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E) . mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M03629-1

27383 MW

58 kDa

Proline-rich AKT1 substrate 1

Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .

T-cell surface glycoprotein CD3 zeta chain

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.