Anti-prioneiwitantilichaam

Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc) . The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease.

Our Prion Protein primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Prion Protein and is protein a purified. It is great for use in ELISA, WB, IHC.

Our Prion Protein primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Prion Protein and is Protein A purified. It is great for use in WB, IHC.

PrP, PrPsc, PrPc

PRNP

P04156

Human

Clone 3F4 was generated from a synthetic peptide taken from amino acids in the central region of human prion protein. This sequence is conserved in feline and hamster, but is less conserved in rat and mouse Prion protein.

Prion Protein

3F4

Unconjugated

ELISA, WB, IHC

Neurodegenerative Disease, Neurogenesis and Development

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

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For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:100

1:1000

The antibody detects endogenous prion protein in human PC3 and A431 cell lines. In addition, the antibody strongly detects human recombinant Prion protein, and weakly detects bovine recombinant Prion protein.

Western blots performed on each lot.

Mouse

Liquid

IgG2a

Human

Quality Control: WB

AB_10632180