Anti-PRR7 Alexa Fluor® 488

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain) . Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Human, Mouse, Rat

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

PRR7

TRAP3/10

ICC, WB (QC tested)

0.1 mg/mL

Alexa Fluor® 488

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286 (22) :19617-29, URL: http://www.ncbi.nlm.nih.gov/pubmed/21460222

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Western blotting: Recommended dilution: 1-5 μg/ml, non-reducing conditions.

Mouse IgG2a