Anti-RAP1GAP Antilichaam Picoband® 31.0647 Vervoeging

Rap1 GTPase-activating protein 1 is an enzyme that in humans is encoded by the RAP1GAP gene. This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor; NHEJ1; XLF

RAP1GAP

5909

P47736

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human RAP1GAP recombinant protein (Position: D11-L661) .

Widely expressed.

Flow Cytometry

Cell Biology, Cell Type Markers, Developmental Biology, Reproduction, Tags & Cell Markers

Immunogen affinity purified.

Liquid

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.

1. Daumke, O., Weyand, M., Chakrabarti, P. P., Vetter, I. R., Wittinghofer, A. The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature 429: 197-201, 2004. 2. Rubinfeld, B., Munemitsu, S., Clark, R., Conroy, L., Watt, K., Crosier, W. J., McCormick, F., Polakis, P. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21-rap1. Cell 65: 1033-1042, 1991. 3. Weiss, J., Rubinfeld, B., Polakis, P. G., McCormick, F., Cavenee, W. K., Arden, K. C. The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-p35. Cytogenet. Cell Genet. 66: 18-21, 1994.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

75187 MW

6

Non-homologous end joining factor 1

Nucleus.

Segment polarity protein dishevelled homolog DVL-1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.