Anti-RFC3 Antibody Picoband® 31.0550 Vervoeging

Replication factor C subunit 3 is a protein that in humans is encoded by the RFC3 gene. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Reversion-inducing cysteine-rich protein with Kazal motifs; hRECK; Suppressor of tumorigenicity 15 protein; RECK; ST15

RFC3

5983

P40938

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human RFC3 recombinant protein (Position: D6-E333) .

Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.

Flow Cytometry

Cancer, Cell Biology, Oncoproteins/Suppressors, Protease Inhibitors, Proteolysis/Ubiquitin, Tumor Suppressors

Immunogen affinity purified.

Liquid

Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.

1. Bowman, G. D., O'Donnell, M., Kuriyan, J. Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature 429: 724-730, 2004. 2. Okumura, K., Nogami, M., Taguchi, H., Dean, F. B., Chen, M., Pan, Z.-Q., Hurwitz, J., Shiratori, A., Murakami, Y., Ozawa, K., Eki, T. Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23. Genomics 25: 274-278, 1995.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Reversion inducing cysteine rich protein with kazal motifs

Cell membrane; Lipid-anchor, GPI-anchor.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.