Anti-RGS14 Antibody Picoband®

Regulator of G-protein signaling 14, also called RGS14, is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.

Boster Bio Anti-RGS14 Antibody catalog # PA2163. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Regulator of G-protein signaling 14; RGS14; RGS14

RGS14

10636

O43566

Rat, Mouse, Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human RGS14, identical to the related mouse and rat sequences.

Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.

WB

G Protein Signaling, Nucleotide Messenger, Second Messenger, Signal Transduction, Signaling Pathway

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Acts as a regulator of G protein signaling (RGS) . Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI) . Confers GDI activity on G (i) alpha subunits GNAI1 and GNAI3, but not G (o) alpha subunit GNAO1 and G (i) alpha subunit GNAI2. Confers GAP activity on G (o) alpha subunit GNAI0 and G (i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF) -stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G (i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G (i) -alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF) -mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. .

1. Kimple, R. J., Kimple, M. E., Betts, L., Sondek, J., Siderovski, D. P. Structural determinants for GoLoco-induced inhibition of nucleotide release by G-alpha subunits. Nature 416: 878-881, 2002. 2. Traver, S., Bidot, C., Spassky, N., Baltauss, T., de Tand, M.-F., Thomas, J.-L., Zalc, B., Janoueix-Lerosey, I., de Gunzburg, J. RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of G-alpha-0. Biochem. J. 350: 19-29, 2000.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA2163

61447 MW

15 kDa

No cross reactivity with other proteins.

Regulator of G-protein signaling 14

Nucleus . Nucleus, PML body . Cytoplasm . Membrane . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Cell projection, dendrite . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule- organizing center (MTOC) . Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Relocalizes to the nucleus in PML nuclear bodies in response to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons. Localizes to spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G (i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS. Colocalizes with G (i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes (By similarity) . .

Contains 1 GoLoco domain.

Regulator of G-protein signaling 14

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.