Anti-RhoA (Ser-188), fosforspecifiek antilichaam

Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDP-bound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) . The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG) . Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.

Our RhoA (Ser-188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat RhoA (Ser-188) and is antigen affinity purified. It is great for use in ELISA, WB.

Our RhoA (Ser-188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat RhoA (Ser-188) and is antigen affinity purified. It is great for use in WB.

RHOA

P06749

Ser-188

Human, Mouse, and Rat

Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat, mouse, and chicken RhoA, but is not found in RhoB or RhoC proteins.

RhoA (Ser-188)

Unconjugated

ELISA, WB

Cell Signaling, Cytoskeletal Regulation, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

22

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2179071