Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband® fluoro594 Vervoeging

Ribonuclease H2 subunit A, also known as RNase H2 subunit A, is an enzyme that in humans is encoded by the RNASEH2A gene. The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2) . RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.

Mucin-20; MUC-20; MUC20; KIAA1359; UNQ2782/PRO7170

RNASEH2A

10535

O75792

Human

No cross-reactivity with other proteins.

E.coli-derived human Ribonuclease H2, subunit A/RNASEH2A recombinant protein (Position: R25-L299) .

Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.

Flow Cytometry

Interleukins

Immunogen affinity purified.

Liquid

May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF) -induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.

1. Coffin, S. R., Hollis, T., Perrino, F. W. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J. Biol. Chem. 286: 16984-16991, 2011. 2. Crow, Y. J., Leitch, A., Hayward, B. E., Garner, A., Parmar, R., Griffith, E., Ali, M., Semple, C., Aicardi, J., Babul-Hirji, R., Baumann, C., Baxter, P., and 33 others. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nature Genet. 38: 910-916, 2006. 3. Frank, P., Braunshofer-Reiter, C., Wintersberger, U., Grimm, R., Busen, W. Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. Proc. Nat. Acad. Sci. 95: 12872-12877, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

108320 MW

6

Mucin 20, cell surface associated

Apical cell membrane. Basolateral cell membrane. Microvillus membrane. Secreted.

Gamma-aminobutyric acid type B receptor subunit 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.