Anti-RNF25 Antilichaam

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD) .

Boster Bio Anti-RNF25 Antibody (Catalog # A10595) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Rnf25, E3 ubiquitin protein ligase RNF25, Ring finger protein 25, RING-type E3 ubiquitin transferase Rnf25, RNF 25, RNF-25

RNF25

Q96BH1

Human, Mouse

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

ELISA, IP, WB

Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Ubiquitin E3 Enzymes

This affinity purified antibody is directed against human RNF25 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.

1.1 mg/mL by UV absorbance at 280 nm

Liquid (sterile filtered)

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity) . Stimulates transcription mediated by NF-kappa-B.

Dry Ice Shipping

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

https://www.bosterbio.com/msds?sku=A10595

51219 MW

E3 ubiquitin-protein ligase RNF25

IgG

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide