Anti-RNF34 Antibody Picoband® fluoro594 Vervoeging

E3 ubiquitin-protein ligase RNF34 is an enzyme that in humans is encoded by the RNF34 gene. The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.

Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8; MED8

RNF34

80196

Q969K3

Human

No cross-reactivity with other proteins.

E.coli-derived human RNF34 recombinant protein (Position: E46-S372) .

Ubiquitous.

Flow Cytometry

Co-Factors, Epigenetics and Nuclear Signaling, Mediator Complex, Pol II Transcription, Polymerase Associated Factors, Transcription

Immunogen affinity purified.

Liquid

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

1. Sasaki, S., Nakamura, T., Arakawa, H., Mori, M., Watanabe, T., Nagawa, H., Croce, C. M. Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer. Oncogene 21: 5024-5030, 2002.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

17136 MW

6

Mediator complex subunit 8

Nucleus .

Chromaffin granule amine transporter

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.