Anti-Robo1 (C-terminaal gebied) Antilichaam

The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.

Our Robo1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Robo1 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our Robo1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Robo1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

ROBO1

Q9Y6N7

Human, Mouse, and Rat

Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.

Robo1 (C-terminal region)

Unconjugated

ELISA, WB, ICC

Cell Signaling, Cytoskeletal Regulation, Neurogenesis and Development

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

180

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

The antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells.

1:200

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_1573625