Anti-Romiplostim (Nplate) ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique. Romiplostim has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Romiplostim will be captured by immobilized Romiplostim. After washing away any unbound substances, a biotin-labeled Romiplostim is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Romiplostim bound in the initial step. The color development is stopped and the intensity of the color is measured.

Romiplostim

Colorimetric

Quantitative

Plasma, Serum

31.25 - 2,000 ng/mL

80-120%

17.08 ng/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.