Anti-RUFY4 Antibody Picoband® fluoro488 Vervoeging

Enables phosphatidylinositol-3-phosphate binding activity. Involved in autophagosome assembly; cellular response to interleukin-4; and positive regulation of macroautophagy. Located in autophagosome.

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

RUFY4

285180

Q6ZNE9

Human, Monkey

No cross-reactivity with other proteins.

E.coli-derived human RUFY4 recombinant protein (Position: M1-K553) .

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Flow Cytometry

Cell Biology

Immunogen affinity purified.

Liquid

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

1. Terawaki, S. , Camosseto, V. , Prete, F. , Wenger, T. , Papadopoulos, A. , & Rondeau, C. , et al. (2015) . Run and fyve domain-containing protein 4 enhances autophagy and lysosome tethering in response to interleukin-4. The Journal of Cell Biology, 210 (7), 1133-1152. 2. Chen, S. , Robert, F. , Lambertus, K. , Yuan, L. , Wang, J. , & Kathryn, R. , et al. (2018) . An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. Nature Genetics. 3. Haenig, C. , Atias, N. , Taylor, A. K. , Mazza, A. , & Wanker, E. E. . (2020) . Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains. Cell Reports, 32 (7), 108050.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

24145 MW

6

Kelch repeat and BTB domain containing 2

Cul3-RING ubiquitin ligase complex.

Transmembrane protein 240

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.