Anti-S100 beta/S100B Antibody Picoband® fluoro488 Vervoeging

S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.

Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100B

S100B

6285

P04271

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta, identical to the related rat and mouse sequences.

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Flow Cytometry

Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, Neural Stem Cells, Stem Cells

Immunogen affinity purified.

Liquid

Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .

1. Wainwright, M. S.; Craft, J. M.; Griffin, W. S. T.; Marks, A.; Pineda, J.; Padgett, K. R.; Van Eldik, L. J. : Increased susceptibility of S100B transgenic mice to perinatal hypoxia-ischemia. Ann. Neurol. 56: 61-67, 2004. 2. Reeves, R. H.; Yao, J.; Crowley, M. R.; Buck, S.; Zhang, X.; Yarowsky, P.; Gearhart, J. D.; Hilt, D. C. : Astrocytosis and axonal proliferation in the hippocampus of S100b transgenic mice. Proc. Nat. Acad. Sci. 91: 5359-5363, 1994.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

10713 MW

No cross reactivity with other proteins.

6

Protein S100-B

Cytoplasm . Nucleus .

Belongs to the S-100 family.

Protein S100-B

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.