Anti-SCF/KITLG Antibody Picoband® fluoro594 Vervoeging

Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117) . SCF can exist both as a transmembrane protein and a soluble protein. SCF is localized to the long arm of human chromosome 12, between 12q14.3 and 12qter. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self renewal and maintenance of HSCs in-vivo. HSCs at all stages of development express the same levels of the receptor for SCF (c-Kit) . The stromal cells that surround HSCs are a component of the stem cell niche, and they release a number of ligands, including SCF.

Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand; Soluble KIT ligand; sKITLG; KITLG; MGF, SCF

KITLG

4254

P21583

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human SCF.

Flow Cytometry

Cytokines, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Innate Immunity, Stem Cells, Surface Molecules

Immunogen affinity purified.

Liquid

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

1. Geissler, E. N., Liao, M., Brook, J. D., Martin, F. H., Zsebo, K. M., Housman, D. E., Galli, S. J. Stem cell factor (SCF), a novel hematopoietic growth factor and ligand for c-kit tyrosine kinase receptor, maps on human chromosome 12 between 12q14.3 and 12qter. Somat. Cell Molec. Genet. 17: 207-214, 1991. 2. Kent D, Copley M, Benz C, Dykstra B, Bowie M, Eaves C (April 2008) . Regulation of hematopoietic stem cells by the steel factor/KIT signaling pathway. Clin. Cancer Res. 14 (7) : 1926–30.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

30899 MW

No cross reactivity with other proteins.

6

Kit ligand

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Belongs to the SCF family.

Kit ligand

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.