Anti-SEMA4A Antibody Picoband® fluoro594 Vervoeging

Semaphorin-4A is a protein that in humans is encoded by the SEMA4A gene. This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF) -mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

PR domain zinc finger protein 5; PR domain-containing protein 5; PRDM5; PFM2

SEMA4A

64218

Q9H3S1

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human SEMA4A recombinant protein (Position: R81-N754) .

Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Flow Cytometry

Cell Adhesion Proteins, ECM Proteins, Neuroscience

Immunogen affinity purified.

Liquid

Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May cause G2/M arrest and apoptosis in cancer cells.

1. Abid, A., Ismail, M., Mehdi, S. Q., Khaliq, S. Identification of novel mutations in the SEMA4A gene associated with retinal degenerative diseases. (Letter) J. Med. Genet. 43: 378-381, 2006. 2. Bryant, L., Lozynska, O., Han, G., Morgan, J. I. W., Gai, X., Maguire, A. M., Aleman, T., Bennett, J. On variants and disease-causing mutations: Case studies of a SEMA4A variant identified in inherited blindness. Ophthalmic Genet. 39: 144-146, 2018. 3. Delgoffe, G. M., Woo, S.-R., Turnis, M. E., Gravano, D. M., Guy, C., Overacre, A. E., Bettini, M. L., Vogel, P., Finkelstein, D., Bonnevier, J., Workman, C. J., Vignali, D. A. A. Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature 501: 252-256, 2013.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

104553 MW

6

PR/SET domain 5

Nucleus.

AP-2 complex subunit beta

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.