Anti-SIAH-interactief eiwit/CACYBP Antibody Picoband® fluoro647 Vervoeging

CACYBP (Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS) . The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

Calcyclin-binding protein; CacyBP; hCacyBP; S100A6-binding protein; Siah-interacting protein; CACYBP; S100A6BP, SIP; PNAS-107

CACYBP

27101

Q9HB71

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences.

In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. .

Flow Cytometry

Neurology Process, Neuroscience

Immunogen affinity purified.

Liquid

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) . .

1. Matsuzawa, S., Reed, J. C. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.Molec. Cell 7: 915-926, 2001. 2. Sun S, Ning X, Liu J, et al. (2007) . Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma.. Biochem. Biophys. Res. Commun. 356 (4) : 864–71.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

26210 MW

No cross reactivity with other proteins.

6

Calcyclin-binding protein

Nucleus . Cytoplasm . Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.

Contains 1 CS domain.

Calcyclin-binding protein (CacyBP/hCacyBP)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.