Anti-Skp1 Monoklonale Antilichaam

Boster Bio Anti-Skp1 Monoclonal Antibody catalog # M00476. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DGCR8; C22orf12, DGCRK6; LP4941

SKP1

6500

P63208

Human, Mouse, Rat

A synthesized peptide derived from human Skp1 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Clone: ADDF-19

Ubiquitously expressed. .

WB, ICC, IF, Flow Cytometry

Cell Biology, F Box E3 Ligase, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, SCF Complex E3 Ligase, Ubiquitin E3 Enzymes

Affinity-chromatography

0.5mg/ml

Liquid

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and s DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri- miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri- miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994) . Specifically recognizes and binds N6- methyladenosine (m6A) -containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998) . Involved in the silencing of embryonic stem cells self-renewal (By similarity) . .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M00476

86045 MW

501 kDa

Microprocessor complex subunit DGCR8

Nucleus . Nucleus, nucleolus . Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC) . Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (PubMed:17159994) .

T-cell surface glycoprotein CD4

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.