Anti-SMCR8 Antibody Picoband® 31.0550 Vervoeging

Enables protein kinase binding activity and protein kinase inhibitor activity. Contributes to guanyl-nucleotide exchange factor activity. Involved in negative regulation of macromolecule metabolic process; regulation of TOR signaling; and regulation of macroautophagy. Located in chromatin; cytoplasm; and nucleoplasm. Part of guanyl-nucleotide exchange factor complex. Colocalizes with Atg1/ULK1 kinase complex.

RNA-binding protein 47; RNA-binding motif protein 47; RBM47

SMCR8

140775

Q8TEV9

Human

No cross-reactivity with other proteins

E.coli-derived human SMCR8 recombinant protein (Position: D86-D776) .

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Flow Cytometry

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

1. Amick, J., Roczniak-Ferguson, A., Ferguson, S. M. C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling. Molec. Biol. Cell 27: 3040-3051, 2016. 2. Bi, W., Yan, J., Stankiewicz, P., Park, S.-S., Walz, K., Boerkoel, C. F., Potocki, L., Shaffer, L. G., Devriendt, K., Nowaczyk, M. J. M., Inoue, K., Lupski, J. R. Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse. Genome Res. 12: 713-728, 2002. 3. Jung, J., Nayak, A., Schaeffer, V., Starzetz, T., Kirsch, A. K., Muller, S., Dikic, I., Mittelbronn, M., Behrends, C. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. eLife 6: e23063, 2017.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

64099 MW

6

RNA-binding protein 47

Nucleus .

Follicular dendritic cell secreted peptide

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.